Part:BBa_K1613023

B0015:I721001

This part is a ligation of B0015 and I721001---the former fragments of K1613017. The description below is from the information of K1613017.

This part is desinged to proceed lead detection in e.coli. When lead ions (Pb2+) enter the e.coli, they will combine with the protein PbrR and then form a dimer. The lead promoter can be controlled by the dimer to further trigger the RFP. The idea of this design came from Brown 2007 igem team, also working on lead detection. We combined I721001 and I721002 with a constitutive promoter J23102 and RFP to make them function.

How many lead ions can get into the cells in different concentrations of lead ions

Whether lead can enter e.coli or not: By using gold nanoparticles(AU-NPs), we measured the lead absorption of e.coli. The results showed that the longer e.coli put in Pb2+ or the higher Pb2+ concentration, the more lead entered the e.coli.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 217